Additional Cell Culture Reagents and Supplements
Sigma Aldrich Fine Chemicals Biosciences CHO Feed Bioreactor Supplement Animal Component-free, solution, sterile-filtered, suitable for cell culture | 1L
CHO Feed Bioreactor Supplement Animal Component-free, solution, sterile-filtered, suitable for cell culture | 1L
Advanced Biomatrix HyStem Version 2.0 Thiol-Modified Hyaluronan Hydrogel Kit, 7.5 mL
HyStem contains thiolated hyaluronic acid for easy 3D cell culture. HyStem version 2.0 is reconstituted with a buffered 1X PBS. The resulting components will also result in a pH neutral isotonic salt hydrogel. The ending pH, osmo, and rheological properties are the same from Version 1.0 to Version 2.0. HyStem hydrogels are xeno-free and completely customizable. The hydrogel is formed when the crosslinking agent, Extralink-Lite (PEGDA) is added to a mixture of Glycosil (thiol-modified hyaluronan). Recommended for applications requiring specific attachment factor optimization as well as non-adherent cell culture, cellular applications. Extracellular matrix proteins can be mixed into the hydrogel and incorporated non-covalently before gelation. The HyStem Hydrogel Kits are optimal for culturing stem cells whose natural environment is rich in hyaluronic acid (HA).
Sigma Aldrich Fine Chemicals Biosciences Biotin | 58-85-5 | MFCD00005541 | 100 mg
Biotin | Purity: 99% | Mol Wt: 244.31 | 58-85-5 | MFCD00005541 | 100 mg
Sigma Aldrich Fine Chemicals Biosciences Insulin solution human, 11061-68-0, MFCD00131380, 5mL
Chemically defined, recombinant, expressed in Saccharomyces cerevisiae, sterile-filtered, BioXtra, suitable for cell culture
Advanced Biomatrix Poly-D-Lysine, Solution, 0.1 mg/mL
Poly-D-Lysine comes with 50 mL of a 0.1 mg/ml sterile-filtered solution. Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule is resistant to enzymatic degradation and has been used to culture a wide variety of cell types. The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment. Poly-D-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions.
BioVendor, LLC Sircol Acid-Salt Wash Reagent (3 x 20 ml), concentrated, Biocolor Item # SWASH
Sircol; Acid-Salt Wash Reagent (3 x 20 ml), concentrated. Contains acetic acid, sodium chloride and surfactants. Dilute the contents of the vial to 100 ml with deionised water before use. Sircol™ Collagen Assay component. Biocolor Item # SWASH
Cell Applications Inc ATTACHMENT FACTOR SOL 100ML
Promote attachment and spreading of endothelial cells. It is required for culturing microvascular endothelial cells 100 ml
Promocell GmbH PromoCell Supplement Pack / Fibroblast Growth Medium 2 Contains: C-37320, C-30310, C-31010
PromoCell Fibroblast Growth Medium 2 is a low-serum (2% V/V) medium and has been developed for the in vitro cultivation of adult Human Fibroblasts.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell lines.To view a list of references where this medium was used with other species/cell types please check manufacturer homepage.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.02 ml / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Sigma Aldrich Fine Chemicals Biosciences Thymidine | 50-89-5 | MFCD00006537 | 25g
Thymidine | MW:242.23 | 50-89-5 | MFCD00006537 | 25g
Biolegend PE anti-mouse/human Bcl-6
PE anti-mouse/human Bcl-6 [IG191E/A8]; Isotype: Mouse IgG1; Reactivity: Human, Mouse; Apps: ICFC; Size: 25 tests
Advanced Biomatrix HyStem-C Version 2.0 Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit, 2.5 mL
HyStem-C contains thiolated hyaluronic acid & gelatin for easy 3D cell culture. HyStem-C version 2.0 is reconstituted with a buffered 1X PBS. The resulting components will also result in a pH neutral isotonic salt hydrogel. The ending pH, osmo, and rheological properties are the same from Version 1.0 to Version 2.0. HyStem-C hydrogels provide an excellent starting point for optimizing the matrix for cell culture. HyStem-C is fully chemically-defined and based on three biocompatible components: thiol-modified hyaluronan (Glycosil), thiol-reactive crosslinker, PEGDA (Extralink), and thiol-modified denatured collagen (Gelin-S). Gelin-S provides basic cell-attachment sites for a wide variety of primary cells and cell lines and is therefore recommended as an ideal substrate for adherent cell types and for cell culture optimization. In some cases, HyStem-C hydrogels can be further enhanced by the addition of ECM proteins to match native signals.
Promocell GmbH PromoCell Supplement Mix / Mammary Epithelial Cell Growth Medium
The PromoCell Mammary Epithelial Cell Growth Medium is a serum-free medium developed for the cultivation of epithelial cells from the mammary gland.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for rat and murine mammary epithelial cells as well as mamma carcinoma cell lines.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vials| Media supplement | Final supplement concentration|| Bovine Pituitary Extract | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 10 ng / ml || Insulin (recombinant human) | 5 μg / ml || Hydrocortisone | 0.5 μg / ml |